The Dynamics 7.1.4 User's Guide is available under Programs, Wyatt Technology, Dynamics 7.1.4.3, Dynamics User's Guide. When the nanoparticles are dispersed in aqueous media, the random Brownian motion results in time-progressive fluctuations of the scattered light under laser beam illuminating. "Dynamic Light Scattering in Evaluating Crystallizability of Macromolecules", Methods in Enzymology 1997, 276, 157-166.Īlso see DynaPro Application Notes under Literature on the Wyatt Technology website at Dynamic light scattering (DLS) is a robust technology to characterize the particle size and size distribution of samples on the nanoscale. Zulauf, Martin D'Arcy, Allan "Light Scattering of Proteins as a Criterion for Crystallization", Journal of Crystal Growth 1992, 122, 102-106.įerre-D'Amare, Adrian Burley, Stephen K. "The Importance of Dynamic Light Scattering in Obtaining Multiple Crystal Forms of Trypanosoma brucei PGK", Protein Science 1998, 7, 504-507. “Light scattering and the absolute characterization of macromolecules”, Analytica Chimica Acta 1993, 272, 1-40.īeretta, Sabrina Lunelli, Lorenzo Chirico, Giuseppe Baldini, Giancarlo "Dynamic Light Scattering from Small Particles: Expected Accuracy in Hemoglobin Data Reduction", Applied Optics 1996, 35(19), 3763-3770.īernstein, Bradley E. "Quasielastic Light Scattering", Analytical Chemistry 1990, 62(20), 1049A-1057A. From the DynaPro NanoStar User’s Guide (M3300 Rev.
#Dls dynamic light scattering software
The scattered light is correlated in the DynaPro NanoStar, which then sends the results to the PC for analysis by the DYNAMICS software package. After the cuvette is placed in the sample cell, the sample is illuminated by the laser. Dynamic light scattering particle size analyzers are used to measure the size of very small particles (0.6 nm to 6 um) in solution. The total sample injection volume (including filtration) is approximately 20 ul using the 10ul cuvette. Solutions are injected into cuvetttes and placed into the sample cell.
The sample is filtered to eliminate dust particles, which might interfere with the signal from the molecules being measured. Knowledge of the underlying physics of molecular sizing is not essential. The DynaPro NanoStar is straightforward to operate.
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